Part:BBa_K563004:Design

pTEF1, promoter of Translational elongation factor EF-1 alpha

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 205
  

Design Notes

We add NcoI and XhoI at the ends of the promoter

Source

Amplify from genome of S.cerevisiae by PCR